coomassie blue recipe

Ponceau S

Ponceau S Acid Red 112 or C I 27195 (systematic name: 3-hydroxy-4-(2-sulfo-4-[4-sulfophenylazo]phenylazo)-2 7-naphthalenedisulfonic acid sodium salt) is a sodium salt of a diazo dye of a light red color that may be used to prepare a stain for rapid reversible detection of protein bands on nitrocellulose or polyvinylidene fluoride (PVDF) membranes (western blotting) as well as on cellulose

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BVDA

The luminol set F-3000 (for 1 litre working solution) is based on a well known and often used recipe (K Weber 1966) The set F-31000 (for 0 5 liter working solution) is on the basis of more concentrated Dutch recipe that was presented on the SPTM conference in Stockholm [3] and the IAFS conference in Los Angeles [4] in 1999 as well as discussed in several articles in the Dutch magazine Modus

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Sauer:bis

We use Coomassie extensively The Coomassie brilliant blue R-250 makes a deep purple color with high contrast The G-250 type makes a softer blue with less contrast We prefer the R-250 Solutions Stain 0 2-0 3 % Coomassie R-250 10 % methanol 10 % acetic acid one liter: Weigh about 2-3 grams of Coomassie and place into a glass bottle

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Lysis buffer (10 ml)

Coomassie blue staining solution 0 1% w/v Coomassie blue R250 40% MeOH 10% HAc Destaining solution 30% MeOH 10% HAc Longer destaining solution 5% MeOH 7 5% HAc 5% glycerol Lysis buffer (10 ml) Final concentrations in the lysis buffer are in parentheses Solutions stored at 4(C

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Life science reagent and buffer recipes

TPE - Recipe for the preparation of Tris-phosphate-EDTA Trypan blue - Recipe for the preparation of trypan blue TSY agar - Trypticase soy yeast agar Recipe TSY broth - Trypticase soy yeast broth Recipe TTE (1X) - Recipe for the preparation of 1X TTE buffer TYG Medium - Tryptone yeast glucose bacterial growth medium

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SimplyBlue SafeStain

SimplyBlue™ SafeStain GelCode Blue Coomassie R-250 Stain After 2 hours in destain After overnight in destain The following samples were electrophoresed on NuPAGE Novex Bis-Tris 4-12% Gels and then stained with either SimplyBlue™ SafeStain GelCode Blue or Coomassie R-250 followingmanufacturer-recommended protocols Items to note are

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3 Isoelectric Focusing (IEF) Protocol

Coomassie Staining Coomassie Blue 250 Staining Total volume /strip Detection limit: 0 5-2ng Detection limit: 5-10ng Detection limit: 30-50ng l 7cm pH4-7 L (linear) 4-8 g 10-20 g 10-60 g 125 7cm pH6-11 L 8-16 g 15-40 g 20-120 g 125 7cm pH3-10 L and pH 3-10 NL (non-linear) 2-4 g 5-10 g 10-60 g 125

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SDSPAGE WesternBlot General Instructions v2

Safety: Coomassie stain is not harmful but care should be taken so as not to stain your clothing Take it from me it is very easy to spill a little bit onto yourself I have a few shirts that are stained blue The SDS-PAGE Sample Buffer contains β-mercaptoethanol This is harmful to breathe in large quantities

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Blue Native Polyacrylamide Gel Electrophoresis (BN

The dye Coomassie blue which binds nonspecifically to all pro-teins and is itself negatively charged is used in BN-PAGE Therefore the electrophoretic mobility of an MPC is determined by the negative charge of the bound Coomassie blue dye and the size and shape of the complex (Fig 1) (1 2) Coomassie blue does

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coomassie blue staining BRIC

Q coomassie blue staining solution에 대한 질문이 있습니다 coomassie blue staining methods에서 각각의 solution에서 들어가는 것들 중에서 methanol과 glacial acetic acid의 grade가 궁금합니다 : A Coomassie 염색에 사용하는 methanol과 acetic acid는 grade와는 상관이 없습니다 덕산에서 깡통으로 판매하는 것을 구매해 사용하면

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Molecular Techniques and Methods Native Gel Electrophoresis

blue purple or red Coomassie Brilliant Blue R250 and PAGE Blue 83 each visibly stain as little as 0 1-1 ug of protein in a band of about 1 cm width NOTES The glycerol in the 2 X Sample Buffer increases the density of the sample to aid the loading of it onto the gel

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Western Blot Buffer (Reagent)

10 11 2012When we do western blot except lysis buffer which is needed in sample preparation other reagents also have to be prepared for western blot Loading buffer running buffer coomassie brilliant blue staining solution and coomassie destaining solutionare needed to be prepared for SDS-PAGE while western blot transfer buffer (recipe here is for wet transfer) preparation

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Blue Native Polyacrylamide Gel Electrophoresis (BN

Coomassie blue does not act as a detergent and it preserves the structure of MPCs In contrast to other native gel electrophoresis systems MPCs are separated independently of their isoelectric point and therefore the size of the MPCs can be estimated (1 7–9) In addition the binding of Coomassie blue to proteins decreases their tendency

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What Is the Function of Tracking Dye in Gel Electrophoresis?

Gel electrophoresis and loading dyes are used when separating large DNA fragments Loading dye purpose and function is to add a color indicator to colorless DNA solutions Dyes help researchers see their sample when pipetting DNA into wells in gel Dyes show how DNA moves during electrophoresis

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Biochem Lab 6 Report greg

Experiment 6 – Lab Report SDS-PAGE/Coomassie Blue Analysis of rGFP fractions This report is worth 40 points and is due at the beginning of your next scheduled laboratory session This report must be typed Questions should be re-typed or restated in your answer To save you time you may use simply edit this word document

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Protein Extraction and Quantification

Coomassie destain [LINK to solution recipe] Coomassie stain [LINK to solution recipe] deferoxamine mesylate salt ‐ DM denaturing buffer [LINK to solution recipe] dimethyl sulfoxide 99 9% ACS spectrophotometric grade ‐ DMSO dithiothreitol ‐ DTT brilliant Blue R 250

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Blue native electrophoresis protocol

Blue native electrophoresis protocol • Coomassie blue G • Vertical acrylamide electrophoresis unit • Electroblotting unit- – fully submerged A recipe for BN -PAGE anode and cathode electrophoresis running buffers are described in the buffer recipes section

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Molecular Techniques and Methods Native Gel Electrophoresis

blue purple or red Coomassie Brilliant Blue R250 and PAGE Blue 83 each visibly stain as little as 0 1-1 ug of protein in a band of about 1 cm width NOTES The glycerol in the 2 X Sample Buffer increases the density of the sample to aid the loading of it onto the gel

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Isoelectric focusing

Isoelectric focusing (IEF) is one of the most commonly used techniques for the separation of proteins IEF separations are based on the pH dependence of the electrophoretic mobilities of the protein molecules Isoelectric focusing makes use of electrical charge properties of molecules to focus them in defined zones in a separation medium It is the []

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The principle and Procedure of Polyacrylamide

Subsequent analysis – Coomassie Blue Staining The gel is rinsed with deionized water 3-5 times to remove SDS and buffer It may create hindrance with the binding of the dye (0 1% Coomassie Blue) to the proteins The gel is then dipped in Coomassie Blue stain (staining buffer) on a shaking incubator at room temperature The invisible bands

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OD P LG H * H OV

The Coomassie Blue Stain Super Destain 5X Running Buffer and 5X Sample Buffer should be prepared before this laboratory experiment is performed Coomassie Blue Stain Preparation Coomassie Blue Stain is prepared using the following recipe: Dissolve 0 25g of Coomassie Brilliant Blue (Bril-liant Blue R 250) into 90 ml of Super Destain Solution

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Ponceau S

Ponceau S Acid Red 112 or C I 27195 (systematic name: 3-hydroxy-4-(2-sulfo-4-[4-sulfophenylazo]phenylazo)-2 7-naphthalenedisulfonic acid sodium salt) is a sodium salt of a diazo dye of a light red color that may be used to prepare a stain for rapid reversible detection of protein bands on nitrocellulose or polyvinylidene fluoride (PVDF) membranes (western blotting) as well as on cellulose

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Blue Native Polyacrylamide Gel Electrophoresis (BN

Coomassie blue does not act as a detergent and it preserves the structure of MPCs In contrast to other native gel electrophoresis systems MPCs are separated independently of their isoelectric point and therefore the size of the MPCs can be estimated (1 7–9)

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