High Throughput Kinetic Assay for Screening Potential

High Throughput Kinetic Assay for Screening Potential Inhibitors of Sickle Hemoglobin Polymerization Abstract Ahmed S Mehanna The current manuscript describes a high throughput assay designed to identify organic compounds with potential inhibitory effects on sickle hemoglobin polymerization The assay is fast economic and reproducible

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Multimode microplate reader Live cell assays

Spark The Spark multimode microplate reader - your research partner Easy selection of one of the four preset configurations for a specific application segment provides a fast solution to solve your research problems The fully configurable platform also gives you

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Kinetic

Product Overview The Kinetic-QCL TM Kinetic Chromogenic LAL Assay is a quantitative kinetic assay for the detection of Gram-negative bacterial endotoxin A sample is mixed with the reconstituted LAL reagent in a 96-well plate and placed in an incubating plate reader that measures absorbance at 405 nm

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Development of a Kinetic Assay for Late Endosome

Development of a Kinetic Assay for Late Endosome Movement: Autoři: EŠNER Milan (203 Česk republika garant domc) Felix MEYENHOFER (756 Švcarsko) Michael KUHN (276 Německo) Melissa THOMAS (276 Německo) Yannis KALAIDZIDIS (276 Německo) a

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JOURNAL OF EVOLUTION OF MEDICAL AND DENTAL

The enzymatic creatinine assay deals effectively with most interfering substances but has a greater cost and shorter shelf-life compared with the kinetic Jaffe's method7 In this study an enzymatic kit method for the determination of serum creatinine was optimized for use with OLYMPUS AU 400Auto analyzer and its performance characteristics and

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A continuous kinetic assay for adenylation enzyme

Steady-state kinetic parameters of the substrate acids were determined for each enzyme using both the hydroxamate-MesG assay and the [32 P]-pyrophosphate exchange assay using the standard assay conditions described above by measuring the initial velocity (v 0) as a function of substrate acid concentration to provide a saturation curve which

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ACE Kinetic – BHLMANN

In cerebrospinal fluid (CSF) normal ACE activity is far below the detection limit of any commercially available ACE kinetic assay BHLMANN offers a solution to reliably detect even this low ACE activity The ACE high sensitive assay (kinetic assay order code: KK-ACF) with a detection limit of 1 U/L

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Lonza™ Kinetic

Zum Nachweis von gramnegativem bakteriellem Endotoxin in humanen und tierischen parenteralen Arzneimitteln biologischen Produkten und medizinischen Gerten Das Lonzatrade Kinetic-QCLtrade Chromogene LAL-Assay-Kit ist ein quantitativer Endotoxintest fr Endprodukte der mit einem Mikrotiterplatten-Lesegert und einer Nachweissoftware verwendet wird

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Lonza Walkersville Kinetic

The Kinetic-QCL Kinetic Chromogenic LAL Assay is a quantitative kinetic assay for the detection of Gram-negative bacterial endotoxin A sample is mixed with the reconstituted LAL reagent in a 96-well plate and placed in an incubating plate reader that measures absorbance at 405 nm

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Salivary Alpha

The Salimetrics Alpha-Amylase Kinetic/Enzymatic Assay Kit is specifically designed and validated for the kinetic measurement of salivary α-amylase activity It is not intended for diagnostic use It is intended only for research use in humans and some animals Salimetrics has not validated this kit for serum or plasma samples Introduction

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HTRF technology

Long signal stability is among the many benefits of HTRF technology HTRF fluorescence is not dampened by assay measurement assay additives such as DMSO or extended incubations prior to reading The advantages of the tremendous stability of HTRF fluorescence include read time flexibility during HTS measurements the ability to perform kinetic studies and increased sample compatibility

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Creatinine Assay Kit

BioAssay Systems' Creatinine Assay Kit (DICT-500) utilizes picrate forming a red colored complex Color intensity at 510nm is proportional to creatinine levels The formulation reduces interference by substances Samples: urine serum plasma biological preparations Detection limit: 0 10 mg/dL8M

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HTRF technology

HTRF fluorescence is not dampened by assay measurement assay additives such as DMSO or extended incubations prior to reading The advantages of the tremendous stability of HTRF fluorescence include read time flexibility during HTS measurements the ability to perform kinetic studies and increased sample compatibility

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Assay Name: Kinetic viability using PI

Celigo Demonstration Experiment – Kinetic viability using PI 2 1001358 Rev B Experiment: Kinetic viability using Propidium Iodide Purpose Perform kinetic viability assay on MDA--231 and K562 cells Current Method(s) Cell Titer Glo Flow Cytometry Target

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Biacore™ Assay Handbook

While the available options for assay development and execution may vary between the different systems the same general principles apply to all systems This book will focus as far as possible on common principles of Biacore assays keeping system-specific information to a minimum Biacore Assay Hbk3 book Page 6 Friday May 4 2012 3:12 PM

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A continuous kinetic assay for adenylation enzyme

Steady-state kinetic parameters of the substrate acids were determined for each enzyme using both the hydroxamate-MesG assay and the [32 P]-pyrophosphate exchange assay using the standard assay conditions described above by measuring the initial velocity (v 0) as a function of substrate acid concentration to provide a saturation curve which was fit by nonlinear regression analysis to the

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Enzyme assay

A kinetic assay in which measurements are performed multiple times over a fixed time interval Kinetic assay results may be visualized numerically (for example as a slope parameter representing the rate of signal change over time) or graphically (for example as a plot of the signal measured at each time point)

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Working with Enzymes: Part I

At the end of my last article I provided some practical tips and tricks for working with enzymes at the bench Now we'll cover one of the cornerstone techniques of enzymology work: the enzyme assay Starting with the simple assays and eventually working our way to the more complex this article introduces the principles of assaying enzymes spectrophotometrically

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Antibiotic microbial assay using kinetic

assay since the incubation is performed in the microplate-spectrophotometer (Brady Katz 1991) This assay em-ploys optical density reading after a given incubation time (endpoint-reading method) The aim of this study was to develop a method for microbiological assay of apramycin employing the kinetic

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