Allogeneic Human Umbilical Cord Mesenchymal Stem

Vials containing only UC‐MSCs were cryopreserved using 6% hydroxyethyl starch (Claris G/LVP‐5 Ellisbridge Ahmedabad India) containing 10% Dimethyl sulfoxide (Sigma D2650 Irvine United Kingdom) first cooled in the −80C ultra‐freezer at approximately 1C/minute from 25C to −80C in a freezing container (Nalgene 5100‐0001 Rochester NY USA) and then plunged directly

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Isolation of Human Endometrial Stromal Cells for In Vitro

Figure 1: Cellular changes of Human endometrial stromal cells during in vitro decidualization HESCs were isolated and cultured for six days in media containing either vehicle or E2 MPA and cAMP (EPC) A) Cell images illustrating the changes in cellular morphology from fibroblastic to epithelioid cells B) Phalloidin stained images of HESC illustrating the cytoskeletal changes (in green

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Safety Data Sheet

Sigma - D2650 Page1 de 7 SIGMA-ALDRICH sigma-aldrich FICHE DE DONNES DE SCURIT conformment au Rglement (CE) No 1907/2006 Version 5 0 Date de rvision 21 04 2012 Date d'impression 16 01 2013 1 IDENTIFICATION DE LA SUBSTANCE/ DU MLANGE ET DE LA SOCIT/ L'ENTREPRISE 1 1 Identificateurs de produit Nom du produit : Dimthylsulfoxyde Code Produit : D2650 Marque : Sigma

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Sigma

FBS Millipore-Sigma USDA Approved: 358 60: 12306C-500ML: Millipore-Sigma USDA Approved Countries Heat Inactivated FBS 500 mL: 375 10: 146153-500G: SILICONE OIL FOR MP BP APPARATUS: D2650-100ML: DIMETHYL SULFOXIDE HYBRI-MAX STERILE 128 04: D2650-5X10ML: DIMETHYL SULFOXIDE HYBRI-MAX STERILE 75 90: D2650-5X5ML: DIMETHYL

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Dmso D2650

Sigma – D2650 Page3 of 8 6 2 Environmental precautions Prevent further leakage or spillage if safe to do so Do not let product enter drains 6 3 Methods and materials for containment and cleaning up 2M DMSO 1M Acetamide 3M Propylene Glycol in PB1 Caution: The solution may become cloudy when DMSO is added DMSO 3 1252 Sigma D2650

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AZD3759 a BBB

Science Translational Medicine 07 Dec 2016: Vol 8 Issue 368 pp 368ra172 DOI: 10 1126/scitranslmed aag0976 Article Figures Data (Sigma D2650) and 0 1% Lutrol F 68 (Poloxamer 188) (BASF 9003-11-6) in saline for erlotinib and 1% methyl cellulose (Sigma 9004-67-5) in water for AZD3759 0 1% Lutrol F 68 in saline was used as the vehicle control In vivo DMPK assay to

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Cryopreservation of hPSCs by Vitrification

Ethylene glycol Sigma 102466 DMSO Sigma D2650 Sucrose Sigma S7903 1M HEPES solution Life Technologies 15630 Cryovials internal threads VWR (Nunc) 66021-986 Cryo storage box Liquid Nitrogen in an ice bucket FREEZING CELLS Prepare Media Prepare all media fresh sterile filter and maintain on ice while working

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Registered report: RAF inhibitors prime wild

16/02/2016Thank you for submitting your work entitled Registered Report: RAF inhibitors prime wild-type RAF to activate the MAPK pathway and enhance growth for peer review at eLife Your submission has been favorably evaluated by Sean Morrison (Senior editor) and five reviewers one of whom is a member of our Board of Reviewing Editors

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The cytotoxic effects of dimethyl sulfoxide in mouse

DMSO (Sigma-Aldrich D2650) was added to KSOM at concentrations of 0% 0 5% 1% and 2% (v/v) Osmolarity levels in each group were determined using the Advanced Model 3320 Micro-Osmometer (Advanced Instrument 3320) according to the manufacturer's instructions Briefly 20 L of each KSOM-DMSO mixture was aspirated by the sampler tip and placed on the instrument's cradle

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Isolation of Mouse Epidermal Keratinocytes and Their

Summary The goal of this protocol is to isolate epidermal keratinocytes from the dorsal skin of adult mice for a variety of downstream applications such as molecular biology biochemistry fluorescence activated cell sorting and primary in vitro uses (e g clonogenic keratinocytes)

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University of Manchester

University of Manchester -Chondrocyte Protocol (Susan Kimber Lab) Cryopreservation medium: Add a 1ml volume of Dimethyl Sulfoxide (Sigma no D2650) to 9ml volume of FBS Pipette up and down to mix well Porcine gelatin (Sigma no G1890) – A 1% stock gelatin solution is prepared by adding 4g of lyophilized gelatin powder to 400ml dH2O The solution is autoclaved to sterilise

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Robust CRISPR/Cas9 Genome Editing of the HUDEP

Robust CRISPR/Cas9 Genome Editing of the HUDEP-2 Erythroid Precursor Line Using Plasmids and Single-Stranded Oligonucleotide Donors Gemma Moir-Meyer 1 Pak Leng Cheong 1 Aude-Anais Olijnik 1 Jill Brown 1 Samantha Knight 2 Andrew King 1 Ryo Kurita 3 Yukio Nakamura 4 Richard J Gibbons 1 Douglas R Higgs 1 Veronica J Buckle 1 and Christian Babbs 1 * Author information

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In vitro expansion and genetic modification of

Dimethyl sulfoxide (DMSO) (SIGMA: D2650)! Caution Harmful Y27632 (RD: 1254) SB431542 (RD: 1614) Stable gene transfection of organoids with lentiviruses 293FT cell line (Invitrogen: R70007) Lentiviral vector plasmid (GFP-expressing) psPAX2 packaging plasmid (Addgene: 12260) pMD2 G envelope plasmid (Addgene: 12259)

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Dimethyl sulfoxide D2650

D2650 Sigma-Aldrich Dimethyl sulfoxide Hybri-Max ™ sterile-filtered BioReagent suitable for hybridoma ≥99 7% Synonym: DMSO CAS Number 67-68-5 Linear Formula (CH 3) 2 SO Molecular Weight 78 13 Beilstein/REAXYS Number 506008 EC Number 200-664-3 MDL number MFCD00002089 PubChem Substance ID 24893703 NACRES NA 75

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ヒトiPSのの

・Dimethyl sulfoxide (DMSO Hybri-Max TM):Sigma D2650 ・チューブ(Nalgene Cryoware TM):NALGENE 5000-0020 ・ディスパーゼ(Roceh Dispase II neutral protease grade II):Roceh 04942078001 ・15mlチューブ ・50mlチューブ ・ Nalgen Cryo1℃Freezing Container #5100-0001、あるいは、 フリーザー

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Buffer Panel Chromatin Immunoprecipitation

• Dimethyl sulfoxide (DMSO Sigma Catalog # D2650 or equivalent) • DNA purification kit (Qiagen Catalog # 28104 or equivalent) • Deionized or distilled water Equipment • Sonicator • Ultrasonic bath • Rocking or shaking device • Eppendorf tube rotator • Benchtop ultracentrifuge • Refrigerated ultracentrifuge • Benchtop centrifuge • Water bath and heatblock

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Isolation of Mouse Epidermal Keratinocytes and Their

Summary The goal of this protocol is to isolate epidermal keratinocytes from the dorsal skin of adult mice for a variety of downstream applications such as molecular biology biochemistry fluorescence activated cell sorting and primary in vitro uses (e g clonogenic keratinocytes)

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PRECISION OF DISPENSING USING VP WANDS AND

Background The method of testing the wands and manifolds are fluorescence-based readings using fluorescein isothiocyanate (FITC) FITC is a derivative of fluorescein with the molecule functionalized with isothiocyanate thus making it reactive towards amine and sulfhydrl groups and a

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Development and improvement of a serum

IS293-V 10% DMSO (Sigma D2650) and 10% Biotech Allentown PA) Dissolved oxygen was main-Bovine Calf Serum tained at 40% of air saturation by a single loop controller The flow rate of air in the headspace was Cell counting and culture monitoring maintained at 0 1 l min The bioreactor tanks were-1

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DIMETHYL SULFOXIDE

Dimethyl sulfoxide (D8779) - Product Information - Sigma-Aldrich Dimethyl sulfoxide (D2650) - Product Information Sheet Dimethyl Sulfoxide (DMSO) - Environmental Health Safety PDF Dimethyl Sulfoxide (DMSO) (NCD 230 12) Dimethyl Sulfoxide (DMSO) Physical Properties Introduction Dimethyl sulfoxide (D2650) - Product Information - Sigma-Aldrich Material Safety Data Sheet DIMETHYL

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Dimethyl Sulfoxide Hybri

Synonym: DMSO Methyl sulfoxide CAS Number: 67-68-5 Linear Formula: (CH 3) 2 SO Molecular Weight: 78 13 Packaging: 5 mL and 10 mL in flame sealed ampules 100 mL in amber bottle Application DMSO is a polar aprotic solvent used in chemical reactions in polymerase chain reactions (PCR) and as a cryoprotectant vitrification agent for the preservation of cells tissues and organs

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Dimethyl sulfoxide Hybri

Dimethyl sulfoxide Hybri-Max sterile-filtered BioReagent suitable for hybridoma 99 7% 5x5ml D2650 SIGMA Fotos Descrio do item: topo Veja tambm: topo ACIDO CLORIDRICO 30% SUPRAPUR 1L 1003181000 MERCK : FLOROGLUCINA 2-HIDRATO PA MERCK 25G 1070690025 MERCK :

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Culturing Protocol for KOMP

EDTA Sigma E6511 Hepes-Buffered D-MEM (for Microinjection media) Gibco 12430-054 D-glucose Sigma G7528 DMSO 100 ml Sigma D2650 Gelatin 2% Sigma G1393 *Alternatively ES Cell Grade FBS may be used e g Gibco 10437028 or Hyclone FBS but serum should always be pre-tested to be ES Cell qualified prior to use

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Standard Operating Procedure (SOP) for

Standard Operating Procedure (SOP) for Peripheral Blood Mononuclear Cells (PBMC) and Plasma Collection for Cell Activation/ Cell Mediated Immunity (CMI) Vaccine and Treatment Evaluation Units (VTEUs) Office of Clinical Research Resources (OCRR) DMID/NIAID/NIH Version 5 0

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6810

Part Alternates: D2650 F92D492 6810-01-360-5697 01-360-5697 6810013605697 013605697 Chemicals and Chemical Products | Chemicals Supply Group (FSG) NSN Assign NIIN Item Name Code (INC) 68: 28 JUN 1992: 01-360-5697: 26874 ( DIMETHYL SULFOXIDE REAGENT0 ) Cross Reference | NSN 6810-01-360-5697 Part Number Cage Code Manufacturer D2650 : 21076: SIGMA CHEMICAL CO: D2650 : Z2W41: SIGMA

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Medium and Reagents for JM8 F6 ES cells

• Add dimethylsulphoxide (DMSO) (Sigma # D2650) to ES cell medium to a final concentration of 10% (v/v) • Filter sterilise Make fresh before use SNL feeder cell medium: • Knockout DMEM • 7% FCS • 1x GPS (Glutamine-Penecillin-Streptomycin) (Antibiotics optional) SNL inactivation medium: • Knockout DMEM • 7% FCS

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