Transient Treatment of Human Pluripotent Stem Cells

Importantly neither 1% or 2% DMSO resulted in cell toxicity as measured by viability counts (n = 3 2D culture % live = control: 80 1 3 1% DMSO: 82 3 7 2%: 81 2 7 3D culture % live = control: 81 4 3 1% DMSO: 82 6 7 2%: 82 2 7) Overall these results are consistent with the notion that DMSO treatment alters the cell cycle and growth patterns in cultured stem cells

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RNA sequencing data of mouse 2

To understand the effect of DMSO in preimplantation embryos we have treated mouse 1 cell zygotes with DMSO and found that DMSO treatment caused 2 or 4 cell embryonic arrest and altered the acetylation levels of mouse preimplantation embryos To illustrate the mechanism of DMSO in mouse preimplantation embryos fertilized zygotes have been treated with 2% of DMSO and then performed RNA-seq

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AID 1258864

BioAssay record AID 1258864 submitted by ChEMBL: LANCE Ultra cAMP Assay: The potency of compounds of the invention as H3 receptor antagonists can be assessed by measuring the blockade of (R)-alpha-methylhistamine-mediated cAMP production utilizing a LANCE Ultra cAMP kit (PE #TRF0263) in CHO cells expressing human H3 receptors (GenBank: BC096840 Strausberg R L et al Proc Natl

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(Hybri

D2650: Product Brand: SIGMA: CAS Number: 67-68-5: Molecular Weight: 78 13 : TEST: SPECIFICATION: Appearance (Turbidity) Clear: Appearance (Colour) Colorless: Appearance (Form) Liquid: Sterility: Pass: Endotoxin Level = 1 EU/ml: Cytoxicity or abnormal cell growth (CF) Not Detected: Purity (GC) = 99 7 %: Water Content (From Raw Material) = 0 1

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Transient Treatment of Human Pluripotent Stem Cells

1 Stem Cell Maintenance NOTE: The cell maintenance protocol described below applies to pluipotent stem cells (PSCs) maintained in an adherent monolayer Media other reagents and cell culture plates used prior to DMSO treatment can be adjusted as needed For all the following protocols in this manuscript cells should be handled under a biological safety cabinet

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Fixable Viability Stain 780 RUO

Bring FVS780 dye powder and 180 μl of fresh cell culture-grade Dimethyl Sulfoxide (DMSO eg Sigma D2650) to room temperature Add 180 μl of DMSO and vortex solution well Inspect the solution and repeat vortex until the stock dye has fully dissolved This is the Stock Solution Storage

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REAGENTS AND MEDIUM PREPARATION

Dimetyl Sulphoxide (DMSO) Sigma # D2650 Medium preparation 10ml: 10ml Volume MEF medium 6ml FBS 2ml DMSO 2ml Add MEF medium and FBS to a 10ml Falcon tube Filter sterilize at 0 22 m Add DMSO Store at +4 C FREEZING MEDIUM I and II FOR HPSC I Freezing medium components: Stock conc Supplier number

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RM N/P for 3 unit : RM2640 50% DMSO

No : SIG-D2650-100ML BUY 3 AT RMRM 2428 802428 80 BUY 5 AT RMRM4048 004048 00 BUY 10 AT RM 8096 00 DMSO OR BTB get 1 FREE PRICE : RM RM219 00219 00 PRICE : RM964 00964 00 Thiazolyl Blue Tetrazolium Bromide No : SIG-M5655-1G PRICE : RMRM 688 00688 00 BUY 5 AT: RMRM342 00342 00 N/P for 5 unit : RM372 SAVE RM30 00 BUY 10 AT: RMRM 670

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DMSO ()methyl sulfoxide、dimethyl

DMSO is also widely utilized in the storage of human and animal cell lines and bacteriophage λ as a cryoprotective agent 4 A protocol to prepare a DMSO solution for freezing cells is as follows: 1) Prepare freezing medium containing culture medium with 10-20% serum and 5-10% DMSO

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Supplementary Materials for

For in vitro studies a stock solution of venetoclax was made in dimethyl sulfoxide (DMSO Sigma D2650) at 50 mM A stock solution of azacitidine (Cayman Chemicals # 11164) was made in DMSO at 200 mM A stock solution of ISRIB (Selleckchem # S7400) was made in DMSO at 8 mM

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Catalog

D2650-100ML Dimethyl sulfoxide Hybri-Max sterile-filtered BioReagent suitable for hybridoma 99 7% 100ml (Sigma-Aldrich) View Detail Add to Order 1 $157 00 Add to Order: DMS666 100 DMS666 100 Virtual DIMETHYL SULFOXIDE (DMSO) Sterile 100ml (BioShop) View Detail Add to Order Delivered in 2 to 3 days (estimated) $80 94 Add to Order: 472301-1L 472301-1L Dimethyl sulfoxide (ACS

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Dimethyl sulfoxide D2650

D2650 Sigma-Aldrich Dimethyl sulfoxide Hybri DMSO (Dimethyl Sulfoxide) is an organosulfur compound with the formula (CH₃)₂SO It is a colorless liquid and is a powerful solvent It dissolves both polar and non-polar compounds This property mak Keywords: Combustion Cryopreservation High performance liquid chromatography Melting Molecular biology Organic synthesis Oxidations

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SIGMA

DMSO is a polar aprotic solvent used in chemical reactions in polymerase chain reactions (PCR) and as a cryoprotectant vitrification agent for the preservation of cells tissues and organs DMSO is used in cell freezing media to protect cells from ice crystal induced mechanical injury It is used for frozen storage of primary sub-cultured and recombinant heteroploid and hybridoma cell lines

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Supplementary Materials for

10% DMSO (Sigma D2650) using 5100 Cryo 1 o freezing containers after 24-72 hours at -80o C cells were transferred to liquid Nitrogen for long-term storage Apheresis samples were obtained through the Hospital of the University of Pennsylvania Apheresis/Infusion Unit and processed by Ficoll gradient purification and frozen as above Viability

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Fixable Viability Stain 780 RUO

Fixable Viability Stain 780 200ug (DMSO eg Sigma D2650) to room temperature Add 180 μl of DMSO and vortex solution well Inspect the solution and repeat vortex until the stock dye has fully dissolved This is the Stock Solution Storage Upon arrival store the dry dye desiccated and protected from light at -80C until use After reconstitution with DMSO store the Stock Solution at

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Dimethyl sulfoxide Hybri

Synonyme: DMSO CAS-Nummer: 67-68-5 Hersteller: SIGMA-ALDRICH Herstellernummer: D2650-100ML Summenformel: C2H6OS Sicherheitsdatenblatt (andere Sprachen auf Anfrage) Zurck zur Homepage Home Nehmen Sie Kontakt auf Kontakt +41 31 932 11 66 infogrogg-chemie ch Dr Grogg Chemie AG - ber uns Einzigartig – Grogg-Shop mit ber 700'000 Artikeln mit den kompletten

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Dimethyl Sulfoxide Hybri

Synonym: DMSO Methyl sulfoxide CAS Number: 67-68-5 Linear Formula: (CH 3) 2 SO Molecular Weight: 78 13 Packaging: 5 mL and 10 mL in flame sealed ampules 100 mL in amber bottle Application DMSO is a polar aprotic solvent used in chemical reactions in polymerase chain reactions (PCR) and as a cryoprotectant vitrification agent for the preservation of cells tissues and organs

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Dimethyl sulfoxide – Elokarsa

Dimethyl sulfoxide DMSO is a polar aprotic solvent used in chemical reactions in polymerase chain reactions (PCR) and as a cryoprotectant vitrification agent for the preservation of cells tissues and organs Details Category: Cryoprotectant Manufacture: Sigma Aldrich Specification grade: Hybri-Max ™ vapor density: 2 7 (vs air) vapor pressure: 0 42 mmHg ( 20 C) sterility: sterile

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Propagation and Maintenance of Mouse N2a Cells

10% DMSO Sigma # D2650 5 x 5ml vials 50% FBS DMEM plus Pen-Strep and L-glutamine Alternative freezing medium: Standard N2a medium plus 10% DMSO This was used to freeze all N2a stocks at Caltech Other reagents used as of 9/2009: Lipofectamine™ LTX and PLUS Reagent Invitrogen #

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A quantitative PCR method for measuring absolute

2011-01-31Prepare 1 litre of the buccal buffer [0 01 M Tris-hydrochloride (Sigma T-3253) 0 1 M ethylenediaminetetraacetic acid tetra Na salt (Sigma E5391) 0 02 M sodium chloride (Sigma S5886)] Thoroughly dissolve the salts IN MilliQ water and make up the volume to 1000 ml Adjust pH to 7 0 and autoclave at 121C for 30 min Buffer will last for up to 3 months when stored as a sterile solution in

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Dmso D2650

Bio Brief: Hybri-Max DMSO – D2650 [VIDEO] Paeonol (purity of 99%) was purchased from Sigma-Aldrich Co (H35803) Pae was dissolved in dimethyl sulfoxide (DMSO Sigma D2650) and stored at −20 C Other reagent sources are listed below: foetal DMSO (catalog # D2650 Sigma) Additional for Freezing: • Knock Out Serum Replacement (catalog # 10828-028 Gibco Life Technologies) referred

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AID 488877

BioAssay record AID 488877 submitted by The Scripps Research Institute Molecular Screening Center: Late stage assay provider counterscreen results from the probe development effort to identify inhibitors of the SARS coronavirus 3C-like Protease (3CLPro): luminescence-based dose-response cell-based assay for restoration of viability of SARS-CoV-infected Vero cells

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